miRNA Profiling












miRNA Profiling

mRNA NCode Products Microarray analysis has become the preferred tool for profiling miRNA expression patterns to gain insight into their relevance in development and disease. The NCode™ miRNA analysis products provide streamlined solutions for the enrichment, amplification, and profiling of miRNA expression with superb sensitivity and reproducibility. This includes leading workflow tools for total RNA isolation, labeling and hybridization, microarrays, controls, data analysis software, and validation with qRT-PCR.

RNA Isolation for miRNA Analysis

Isolate total RNA from cells or tissue in less than one hour using TRIzol® in a single step method.

 

miRNA Fluorescent Labeling

A sensitive and accurate system for directly labeling endogenous miRNAs using minimal starting material.


miRNA Amplification

Perform consistent and accurate >1,000-fold amplification for miRNA profiling.

miRNA Microarrays

Multispecies arrays corresponding to Sanger miRBase 9.0, spotted in triplicate with extensive controls for hybridization specificity.


miRNA qRT-PCR

An optimized polyadenylation reaction with SuperScript® III RT, the market-leading reverse transcriptase, in a first strand cDNA synthesis reaction.


miRNA Experimental Planning and Design

New to miRNA research?